Immunogen

Recombinant protein corresponding to the Human wild type Transthyretin.
GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKT
SESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEH
AEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

The epitope has been mapped to residue 56-61

Host

Mouse

Clonality

Monoclonal

Subclass/Isotype

IgG1

Purity

Affinity Purified

Format

Lyophilized

Quantity

100 μg

Reconstitution

Add 100 ul PBS to reconstitute to 1 mg/ml 

Storage

Store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

The antibody performs well in both ELISA, IHC and Western blot. Dry milk dissolved in TBS containing 0.3% Tween-20 provides a good blocking and incubation solution. Washing can preferably be performed using TBS containing 0.3% tween-20. 

Recommended dilution

1:1000 (ELISA), 1:1000 (Western Blot) 1:500 (IHC)

Expected | apparent MW

155 kDa

Confirmed Reactivity

Human Transthyretin Amyloids only

Predicted Reactivity

No confirmed exceptions from predicted reactivity are currently known.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Application Examples

Western analysis

Membrane: PVDF
Blocking buffer: 1% dry milk in TBS, 0.3% Tween 20
Antibody dilution : 1:1000
Secondary antibody: anti-mouse (HRP)
Detection: Enhanced Chemoluminescence (pico) 150s

Additional information

Specifically reactive to the amyloid form of human Transthyretin. Epitope mapped to residue 56-61 which remains buried within the native fold of transthyretin but becomes exposed within its amyloid form.

It has been suggested that that two distinct mechanisms of TTR-amyloidosis exists. The first, most common seen in wild type TTR Amyloidosis, consists of the full length TTR. Whereas the other type of amyloidosis mainly consists of the C-terminal region of the protein and is more common in mutant versions of TTR. Mouse IgG1 Anti-Transthyretin 56-61 (Amyloid Specific) epitope is located at the C-terminal strand of cleaved TTR and is suitable to detect amyloid formation derived from the C-terminal.