EGFR抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-10007R
英文名稱EGFR
中文名稱表皮生長因子受體抗體
別 名EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Avian erythroblastic leukemia viral (verbb) oncogene homolog; EGF R; EGFR; Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; mENA; Oncogene ERBB; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN
規(guī)格價(jià)格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價(jià)
說 明 書50ul 100ul 200ul
研究領(lǐng)域腫瘤 細(xì)胞生物 **學(xué) 細(xì)胞膜受體
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep,
EGFR抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:200-800 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
EGFR抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
EGFR抗體溶解方法：
1、在我們公司購買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlEGFR抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí)，再使用對應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。
EGFR抗體關(guān)聯(lián)產(chǎn)品列表：
IC588Mu013 尿溶蛋白1A(UPK1A)重組蛋白 Recombinant Uroplakin 1A (UPK1A) Mus musculus (Mouse)
IC588Mu021 M2-型丙酮酸激酶(PKM2)重組蛋白 Recombinant Pyruvate Kinase, Muscle (PKM2) Mus musculus (Mouse)
IC588Ra011 M2-型丙酮酸激酶(PKM2)重組蛋白 Recombinant Pyruvate Kinase, Muscle (PKM2) Rattus norvegicus (Rat)
IC588Ra012 信號轉(zhuǎn)導(dǎo)銜接分子1(STAM1)重組蛋白 Recombinant Signal Transducing Adaptor Molecule 1 (STAM1) Rattus norvegicus (Rat)
IC589Hu011 血紅素氧合酶2(HO2)重組蛋白 Recombinant Heme Oxygenase 2, Decycling (HO2) Homo sapiens (Human)
IC589Hu012 干擾素調(diào)節(jié)因子3(IRF3)重組蛋白 Recombinant Interferon Regulatory Factor 3 (IRF3) Homo sapiens (Human)
IC589Hu013 微纖絲關(guān)聯(lián)蛋白4(MFAP4)重組蛋白 Recombinant Microfibrillar Associated Protein 4 (MFAP4) Homo sapiens (Human)
IC589Hu014 非受體型蛋白酪氨酸磷酸酶6(PTPN6)重組蛋白 Recombinant Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) Homo sapiens (Human)
IC589Hu015 泛醇細(xì)胞色素C還原酶結(jié)合蛋白(UQCRB)重組蛋白 Recombinant Ubiquinol Cytochrome C Reductase Binding Protein (UQCRB) Homo sapiens (Human)
IC589Mu011 干擾素調(diào)節(jié)因子3(IRF3)重組蛋白 Recombinant Interferon Regulatory Factor 3 (IRF3) Mus musculus (Mouse)
IC589Mu012 血紅素氧合酶2(HO2)重組蛋白 Recombinant Heme Oxygenase 2, Decycling (HO2) Mus musculus (Mouse)
IC589Mu021 干擾素調(diào)節(jié)因子3(IRF3)重組蛋白 Recombinant Interferon Regulatory Factor 3 (IRF3) Mus musculus (Mouse)
IC589Ra011 血紅素氧合酶2(HO2)重組蛋白 Recombinant Heme Oxygenase 2, Decycling (HO2) Rattus norvegicus (Rat)
IC589Ra012 微纖絲關(guān)聯(lián)蛋白4(MFAP4)重組蛋白 Recombinant Microfibrillar Associated Protein 4 (MFAP4) Rattus norvegicus (Rat)
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