IGF 1抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶(hù)反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬(wàn)種科研用專(zhuān)業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國(guó)75%以上現(xiàn)貨。

產(chǎn)品編號(hào)bs-0014R

英文名稱(chēng)IGF 1

中文名稱(chēng)胰島素樣生長(zhǎng)因子1抗體

別    名IBP1; IGF 1; IGF I; IGF-1;IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Full=Insulin-like growth factor I; Insulin Like Growth Factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; MGF; Mechano growth factor; MGF; Somatomedia C; Somatomedin C; IGF1_HUMAN.  IGF-I;

JournalPMIDIFApplicationBiotechnic & Histochemistry (2013)234802281.0630IHCBiotechnic & Histochemistry (2014)244765631.0630IHC-P

規(guī)格價(jià)格50ul/780元 購(gòu)買(mǎi)    100ul/1280元 購(gòu)買(mǎi)    200ul/1980元 購(gòu)買(mǎi)    大包裝/詢(xún)價(jià)

說(shuō) 明 書(shū)50ul  100ul  200ul

研究領(lǐng)域**學(xué)  發(fā)育生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  生長(zhǎng)因子和**  糖尿病  

抗體來(lái)源Rabbit

克隆類(lèi)型Polyclonal

交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,

IGF 1抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量177kDa

細(xì)胞定位細(xì)胞漿

性    狀Lyophilized or Liquid

濃    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Nestin

亞    型IgG

純化方法affinity purified by Protein A

儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

IGF 1抗體產(chǎn)品介紹background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

IGF 1抗體溶解方法：

1、在我們公司購(gòu)買(mǎi)抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml

2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無(wú)需再溶解）：

溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；

若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。

溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）

的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。

3、抗體的分裝

抗體溶解后，將其分裝成 5-10支（以0.1mlIGF 1抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。

另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋?zhuān)绻尘案撸€可再稀釋?zhuān)x一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。

后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。

我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。

IGF 1抗體關(guān)聯(lián)產(chǎn)品列表：

bs-0065R YES 合格 Substance P P物質(zhì)抗體
bs-0066R No 合格 合格 TGF alpha 轉(zhuǎn)移生長(zhǎng)因子α抗體
bs-0067R YES 合格 NGFB 神經(jīng)生長(zhǎng)因子β抗體
bs-0074R No 合格 NFkB Inducing Kinase NIK NFkB誘導(dǎo)激酶抗體
bs-0076M YES 合格 beta-Amyloid 1-42 (CT) β淀粉樣肽1-42(C端)/β-Amyloid 1-42 (CT)抗體
bs-0076R No 合格 合格 beta-Amyloid 1-42(CT) β淀粉樣肽/Aβ42抗體 
bs-0079R YES 合格 合格 CD19 CD19抗體
bs-0080R No 合格 CD20 CD20抗體
bs-0081R YES 合格 合格 合格 Caspase-3 活化半胱胺酸蛋白酶蛋白-3抗體
bs-0084R No 合格 Caspase-6 subunit p18 半胱胺酸蛋白酶蛋白-6抗體
bs-0086R YES 合格 合格 合格 TGF beta 1 轉(zhuǎn)化生長(zhǎng)因子β1/TGF β1/TGF-β1抗體
bs-0087R No 合格 合格 caspase-3 p12 subunit 活化半胱胺酸蛋白酶蛋白3抗體
bs-0088R No 合格 Secretin 分泌素抗體
bs-0092R No 合格 Caspase-10 半胱胺酸蛋白酶-10抗體
bs-0095R YES 合格 Calponin 1 鈣調(diào)節(jié)蛋白-1抗體
bs-0096R YES 合格 alpha 1 Antitrypsin α-1抗胰蛋白酶抗體
bs-0097R No 合格 ALK 間變型**瘤激酶抗體
bs-0099R No 合格 TGF beta 3 propeptide 轉(zhuǎn)移生長(zhǎng)因子β3抗體（TGFβ3）