HPV16 E6 protein抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶(hù)反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬(wàn)種科研用專(zhuān)業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國(guó)75%以上現(xiàn)貨。
產(chǎn)品編號(hào)bs-0990R
英文名稱(chēng)HPV16 E6 protein
中文名稱(chēng)人類(lèi)**狀瘤病毒16抗體
別 名E6; HPV16 E6 protein; Human Papilloma Virus; Human papillomavirus type 16 E6; Human papillomavirus type 16; Protein E6; E6 protein; HPV16 E6; HPV16-E6; VE6_HPV16.
規(guī)格價(jià)格50ul/780元 購(gòu)買(mǎi) 100ul/1280元 購(gòu)買(mǎi) 200ul/1980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià)
說(shuō) 明 書(shū)50ul 100ul 200ul
研究領(lǐng)域腫瘤 **及病毒
抗體來(lái)源Rabbit
克隆類(lèi)型Polyclonal
交叉反應(yīng) Human, HPV16
HPV16 E6 protein抗體產(chǎn)品應(yīng)用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
HPV16 E6 protein抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
HPV16 E6 protein抗體溶解方法：
1、在我們公司購(gòu)買(mǎi)抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無(wú)需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlHPV16 E6 protein抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋?zhuān)绻尘案?，還可再稀釋?zhuān)x一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。
HPV16 E6 protein抗體關(guān)聯(lián)產(chǎn)品列表：
IC585Mu024 尿皮質(zhì)素2(UCN2)重組蛋白 Recombinant Urocortin 2 (UCN2) Mus musculus (Mouse)
IC585Ra011 細(xì)胞周期素D1(CCND1)重組蛋白 Recombinant Cyclin D1 (CCND1) Rattus norvegicus (Rat)
IC585Ra012 尿皮質(zhì)素2(UCN2)重組蛋白 Recombinant Urocortin 2 (UCN2) Rattus norvegicus (Rat)
IC586Hu011 細(xì)胞周期素D2(CCND2)重組蛋白 Recombinant Cyclin D2 (CCND2) Homo sapiens (Human)
IC586Hu012 線(xiàn)粒體解偶聯(lián)蛋白2(UCP2)重組蛋白 Recombinant Uncoupling Protein 2, Mitochondrial (UCP2) Homo sapiens (Human)
IC586Hu013 肌纖蛋白(MYOC)重組蛋白 Recombinant Myocilin (MYOC) Homo sapiens (Human)
IC586Hu014 整合素β2(ITGβ2)重組蛋白 Recombinant Integrin Beta 2 (ITGb2) Homo sapiens (Human)
IC586Hu021 整合素β2(ITGβ2)重組蛋白 Recombinant Integrin Beta 2 (ITGb2) Homo sapiens (Human)
IC586Mu011 線(xiàn)粒體解偶聯(lián)蛋白2(UCP2)重組蛋白 Recombinant Uncoupling Protein 2, Mitochondrial (UCP2) Mus musculus (Mouse)
IC586Mu012 整合素β2(ITGβ2)重組蛋白 Recombinant Integrin Beta 2 (ITGb2) Mus musculus (Mouse)
IC586Mu013 細(xì)胞周期素D2(CCND2)重組蛋白 Recombinant Cyclin D2 (CCND2) Mus musculus (Mouse)
IC586Ra011 線(xiàn)粒體解偶聯(lián)蛋白2(UCP2)重組蛋白 Recombinant Uncoupling Protein 2, Mitochondrial (UCP2) Rattus norvegicus (Rat)
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