Survivin抗體經ICC, IHC, WB, FACS嚴格驗證,品質保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術支持幫您更快得到實驗結果,現貨.我司提供8萬種科研用專業(yè)抗體,經嚴格質控,特異性高,可重復性強,驗證數..可與小鼠, 大鼠, 人樣本反應并經WB, IHC, ICC實驗嚴格驗證。所有產品均提供質保服務,中國75%以上現貨。
產品編號bs-0615R
英文名稱Survivin
中文名稱細胞凋亡抑制因子抗體
別 名API4; API-4; API 4; Survivin variant 3 alpha; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC-5; EPR 1; EPR-1; IAP4; IAP-4; IAP 4; SVV; TIAP; BIRC5_HUMAN.
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領域腫瘤 細胞生物 **學 細胞凋亡
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
Survivin抗體產品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Survivin抗體產品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Survivin抗體溶解方法:
1、在我們公司購買抗體后,查看標簽上的規(guī)格,如:0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加0.1ml雙蒸水到試管內,混勻,-20℃保存;
若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加與標簽上規(guī)格一致的(如:0.2ml)雙蒸水溶解,混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規(guī)格的 50%)雙蒸水到試管內,再加 0.05ml(規(guī)格的 50%)
的甘油到試管內,混勻,-20℃保存; 若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加標簽上規(guī)格的 50%(如:0.1ml)雙蒸水,再加標簽上規(guī)格的50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
3、抗體的分裝
抗體溶解后,將其分裝成 5-10支(以0.1mlSurvivin抗體為例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反復凍溶。
另:預試驗可做幾個梯度1:100、1:200、1:400稀釋,如果背景高,還可再稀釋,選一個*佳的稀釋比例,再正式做,效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時,再使用對應的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個月有效;若在-20℃下保存(推薦),至少三年有效。
Survivin抗體關聯產品列表:
bs-1523R YES 合格 合格 CD63 黑色素瘤相關抗原抗體
bs-15241R No 合格 C6orf222 6號染色體開放閱讀框222抗體
bs-15243R No 合格 C6orf225 6號染色體開放閱讀框225抗體
bs-15247R No 合格 C6orf50 6號染色體開放閱讀框50抗體
bs-1524R No 合格 GlyR alpha 1 + 2 + 3 甘氨酸受體alpha 1/2/3型抗體
bs-15251R No 合格 C6orf62 6號染色體開放閱讀框62抗體
bs-15252R No 合格 C6orf70 6號染色體開放閱讀框70抗體
bs-15258R No 合格 C7orf13 7號染色體開放閱讀框13抗體
bs-15260R No 合格 C7orf25 7號染色體開放閱讀框25抗體
bs-15262R No 合格 C7ORF29 7號染色體開放閱讀框29抗體
bs-15267R No 合格 C7ORF43 7號染色體開放閱讀框43抗體
bs-15269R No 合格 C7orf44 7號染色體開放閱讀框44抗體
bs-15270R No 合格 C7orf45 7號染色體開放閱讀框45抗體
bs-15274R No 合格 C7orf57 7號染色體開放閱讀框57抗體
bs-15277R No 合格 C7orf62 7號染色體開放閱讀框62抗體
bs-15278R No 合格 C7orf63 7號染色體開放閱讀框63抗體
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