Visfatin抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質(zhì)保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實驗結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質(zhì)控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質(zhì)保服務,中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0272R
英文名稱Visfatin
中文名稱內(nèi)脂素/內(nèi)臟脂肪素/前B細胞克隆增強因子1抗體
別 名pre-B-cell colony-enhancing factor 1; PBEF1 protein; AI480535; 1110035O14Rik; AI314458; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Visfatin; NAMPT_HUMAN.
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領域心血管 神經(jīng)生物學 糖尿病 ***病
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Monkey,
Visfatin抗體產(chǎn)品應用WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Visfatin抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Visfatin抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlVisfatin抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
Visfatin抗體關聯(lián)產(chǎn)品列表：
bs-13955R No 合格 CILP2 軟骨中間層蛋白CILP2抗體
bs-13960R No 合格 GEP100/IQSEC1 ADP-核糖基化因子鳥嘌呤核苷酸交換蛋白100抗體
bs-13964R No 合格 Collagen XVII/BP180 XVII型膠原蛋白/膠原蛋白17/17型膠原蛋白抗體
bs-13968R No 合格 CHST7 碳水化合物磺基轉(zhuǎn)移酶7抗體
bs-13969R No 合格 Complement factor 8 beta 補體C8β鏈抗體
bs-1396R YES 合格 合格 CEBP Beta 轉(zhuǎn)錄調(diào)節(jié)因子C/EBP β抗體
bs-13975R No 合格 CD44v10 CD44V10抗體
bs-13976R No 合格 phospho-COP1 (Ser387) 磷酸化E3泛素蛋白連接酶COP1抗體
bs-13978R No 合格 COPG 外殼蛋白COPG-γ抗體
bs-13979R No 合格 合格 COPG2 外殼蛋白γ2-COP抗體
bs-13983R No 合格 COPZ2 衣殼蛋白-ζ2抗體
bs-13984R No 合格 COQ2 線粒體COQ2抗體
bs-1398R No 合格 ELK1 細胞轉(zhuǎn)錄因子ELK1抗體
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