Big endothelin-1抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質(zhì)保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實驗結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質(zhì)控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0188R
英文名稱Big endothelin-1
中文名稱內(nèi)皮素-1抗體
別 名EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP; PPET1; Preproendothelin 1; Preproendothelin1.
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領(lǐng)域心血管 神經(jīng)生物學
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat,
Big endothelin-1抗體產(chǎn)品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Big endothelin-1抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Big endothelin-1抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlBig endothelin-1抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
Big endothelin-1抗體關(guān)聯(lián)產(chǎn)品列表：
bs-12741R No 合格 PMM2 磷酸甘露糖變位酶2抗體
bs-12744R No 合格 PNMA5 類腫瘤性抗原MA5抗體
bs-12745R No 合格 PNPLA3 脂肪營養(yǎng)素PNPLA3抗體
bs-1274R No 合格 Integrin alpha E 整合素αE抗體
bs-12750R No 合格 PODNL1 PODN樣蛋白1抗體
bs-12751R No 合格 POFUT2 蛋白O巖藻糖基轉(zhuǎn)移酶2抗體
bs-12755R No 合格 POLD3 DNA聚合酶α3抗體
bs-1275R No 合格 FoxP1 叉頭蛋白P1抗體
bs-1276R No 合格 DNAJC10 Erdj5/DNAJC10蛋白抗體
bs-12770R No 合格 VPS29 液泡蛋白分選蛋白29抗體
bs-12771R No 合格 VPS33B 液泡蛋白分選蛋白33B抗體
bs-12773R No 合格 VPS36 液泡蛋白分選蛋白36抗體
bs-12777R No 合格 VPS45A 液泡蛋白分選蛋白45A抗體
bs-12778R No 合格 VPS4B 液泡蛋白分選蛋白4B抗體
bs-1277R YES 合格 CFTR 囊性纖維化跨膜轉(zhuǎn)運調(diào)節(jié)因子抗體
- 溫馨提示：為規(guī)避購買風險，建議您在購買前務(wù)必確認供應商資質(zhì)與產(chǎn)品質(zhì)量。
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