TGF beta 3 propeptide抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實驗結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質(zhì)控,特異性高,可重復(fù)性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0099R
英文名稱TGF beta 3 propeptide
中文名稱轉(zhuǎn)移生長因子β3抗體（TGFβ3）
別 名TGF beta 3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; TGFB3; TGFB3_HUMAN; transforming growth factor beta 3; Transforming growth factor beta-3.
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領(lǐng)域信號轉(zhuǎn)導(dǎo) 干細胞 生長因子和**
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Rabbit,
TGF beta 3 propeptide抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
TGF beta 3 propeptide抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
TGF beta 3 propeptide抗體溶解方法：
1、在我們公司購買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlTGF beta 3 propeptide抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
TGF beta 3 propeptide抗體關(guān)聯(lián)產(chǎn)品列表：
bs-10680R YES 合格 NF-H 高分子量神經(jīng)絲蛋白抗體
bs-1068R No 合格 NMDAR1 離子型谷氨酸受體1抗體
bs-10696R No 合格 BMP2 骨形態(tài)發(fā)生蛋白2抗體
bs-10699R No 合格 合格 合格 CD8 CD8抗體
bs-10701R No 合格 HS6ST1 硫酸乙酰肝素6腦苷脂轉(zhuǎn)硫酸酶1抗體
bs-10704R No 合格 NELL1 蛋白激酶C結(jié)合蛋白NELL1抗體
bs-10709R No 合格 I-309/CCL1 嗜酸粒細胞趨化蛋白CCL1抗體
bs-10724R No 合格 AKT2 蛋白激酶B2抗體
bs-10727R No 合格 CD101 CD101抗體
bs-10728R No 合格 RhD Rh polypeptide 2抗體
bs-10738R No 合格 SLC19A2 SLC19A2抗體
bs-10742R No 合格 LPCAT2 磷脂酰基轉(zhuǎn)移酶2抗體
bs-10743R No 合格 合格 Caspase-1 p20 天冬氨酸-胱氨酸特異性蛋白酶家族1 P20抗體
bs-10750R No 合格 C1Q 補體C1qα鏈多肽抗體
bs-10751R No 合格 合格 Sialoadhesin 唾液酸結(jié)合性**球蛋白樣凝集素1抗體
bs-10752R No 合格 GARS X連鎖Charcot-Marie-Tooth神經(jīng)病變相關(guān)蛋白抗體
bs-10755R No 合格 CaIPLA2 胞漿鈣獨立磷脂酶A2抗體
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