Calponin 1抗體經ICC, IHC, WB, FACS嚴格驗證,品質保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術支持幫您更快得到實驗結果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經嚴格質控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經WB, IHC, ICC實驗嚴格驗證。所有產品均提供質保服務,中國75%以上現(xiàn)貨。
產品編號bs-0095R
英文名稱Calponin 1
中文名稱鈣調節(jié)蛋白-1抗體
別 名Basic calponin; Calponin 1; Calponin-1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin1; Calponins basic; CNN 1; CNN1; Sm Calp; SMCC; CNN1_HUMAN; Calponin H1; Calponin1; Sm Calp; smooth muscle.
JournalPMIDIFApplicationMolecular Endocrinology (2014)253214134.7460IHC-PTissue Engineering (2014)244176864.0650IHC-F
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領域心血管 信號轉導 干細胞 細胞骨架
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,
Calponin 1抗體產品應用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Calponin 1抗體產品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Calponin 1抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlCalponin 1抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
Calponin 1抗體關聯(lián)產品列表：
bs-1050R YES 合格 合格 Connexin-40 間隙連接蛋白40抗體
bs-1052R No 合格 NAP1L1 核小體組裝蛋白1抗體
bs-10539R No 合格 合格 TTF1 甲狀腺核轉錄因子-1抗體
bs-10540R No 合格 IL-1 Alpha Propeptide 白介素1α前肽/IL-1α前肽抗體
bs-10549R No 合格 CD55 衰變加速因子CD55抗體
bs-10556R No 合格 SLC27A1 長鏈脂肪酸轉運蛋白1抗體
bs-10557R No 合格 GOT2 谷草轉氨酶2抗體
bs-10558R No 合格 beta Amyloid 1-16 β淀粉樣肽1-16/Aβ1-16抗體
bs-10559R No 合格 beta Amyloid 1-28 β淀粉樣肽1-28/β-Amyloid 1-28抗體
bs-1056R YES 合格 5HT2A Receptor 5-羥色胺受體2A抗體
bs-10574R No 合格 Centrin 1 中心體蛋白1抗體
bs-10578R No 合格 WASF3 Verprolin同源結構域包含蛋白3抗體
bs-10581R No 合格 合格 MMP13 基質金屬蛋白酶13抗體
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