TGF beta 1抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實驗結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質(zhì)控,特異性高,可重復(fù)性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-20411R
英文名稱TGF beta 1 (Cleaved)
中文名稱轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體
別 名Cleaved TGF beta 1 ; CED; DPD1; LAP; Latency-associated peptide; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1.
規(guī)格價格100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書100ul 200ul
研究領(lǐng)域心血管 信號轉(zhuǎn)導(dǎo) 生長因子和** 轉(zhuǎn)錄調(diào)節(jié)因子
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Rabbit, Sheep, Guinea Pig,
TGF beta 1抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
TGF beta 1抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
TGF beta 1抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlTGF beta 1抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
TGF beta 1抗體關(guān)聯(lián)產(chǎn)品列表：
bs-10409R No 合格 CCL26 Signal peptide 嗜酸粒細胞趨化蛋白3抗體
bs-1040R No 合格 factor V 凝血因子5抗體
bs-10410R YES 合格 chemerin 趨化素抗體
bs-10412R YES 合格 VEGFR2 血管內(nèi)皮生長因子受體2抗體
bs-10413R No 合格 Ghrelin 28 (n-octanoyl Ser26) 辛?；X腸肽抗體
bs-10414R No 合格 Nanog 胚胎干細胞關(guān)鍵蛋白抗體
bs-10419R No 合格 合格 GPI 糖磷脂酰肌醇抗體
bs-1041R No 合格 MEK1 + MEK2 絲裂原活化蛋白激酶激酶1抗體
bs-10423R YES 合格 合格 Collagen I I型膠原蛋白/膠原蛋白1/1型膠原蛋白/I型膠原a1抗體
bs-10428R No 合格 C2a 補體C2a鏈多肽抗體
bs-10429R No 合格 beta Amyloid 1-28 β淀粉樣肽1-28/β-Amyloid 1-28抗體
bs-10430R No 合格 CD90 CD90抗體
bs-10432R No 合格 CACNB3 L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體
bs-10434R No 合格 NR0B1 腎上腺發(fā)育不全相關(guān)蛋白抗體
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