Insulin Receptor alpha抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號(hào)bs-0260R
英文名稱Insulin Receptor Alpha
中文名稱胰島素受體α抗體
別 名CD220; CD220 antigen; HHF5; HIR A; INSR alpha; INSR; Insulin receptor; Insulin receptor subunit alpha; IR; INSR_HUMAN.
規(guī)格價(jià)格100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價(jià)
說 明 書100ul 200ul
研究領(lǐng)域細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 激酶和磷酸酶 細(xì)胞膜受體 糖尿病
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,
Insulin Receptor alpha抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Insulin Receptor alpha抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Insulin Receptor alpha抗體溶解方法：
1、在我們公司購買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlInsulin Receptor alpha抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。
Insulin Receptor alpha抗體關(guān)聯(lián)產(chǎn)品列表：
bs-0719R YES 合格 CEA 癌胚抗原抗體
bs-0725R YES 合格 Estrogen Receptor alpha 雌**受體α抗體
bs-0730R YES 合格 HSP27 熱休克蛋白27/HSP25抗體
bs-0732R YES 合格 合格 Cyclooxygenase 2 環(huán)氧合酶2/前列腺素內(nèi)過氧化物合成酶2抗體
bs-0733R No 合格 ADAMTS7(NT) 整合素樣金屬蛋白酶與凝血酶1型-7抗體 (N端抗體)
bs-0734R YES 合格 KGF 纖維母細(xì)胞生長因子7抗體
bs-0735R YES 合格 FGF8 成纖維細(xì)胞生長因子8抗體
bs-0736R YES 合格 CD56 神經(jīng)細(xì)胞粘附分子1抗體
bs-0737R YES 合格 HIF-1 Alpha 缺氧誘導(dǎo)因子1α 抗體HIF-1α
bs-0738R YES 合格 Livin 凋亡抑制蛋白抗體
bs-0740R No 合格 CDKN2A/p16-INK4a 抑癌基因p16抗體
bs-0741R YES 合格 CDKN1A/p21 p21蛋白抗體
bs-0742R YES 合格 合格 CDKN1B/p27 KIP 1 P27抗體/周期素依賴激酶抑制劑
bs-0747R YES 合格 合格 RANKL/CD254 骨保護(hù)蛋白配體/破骨細(xì)胞分化因子抗體
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