P53 protein抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬(wàn)種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國(guó)75%以上現(xiàn)貨。

產(chǎn)品編號(hào)bs-0033R

英文名稱P53 protein(wt-p53)

中文名稱腫瘤抑制基因P53蛋白/野生型P53抗體

別    名Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53.  

JournalPMIDIFApplicationJournal of Biological Chemistry (2015)264682764.6000WBBMC Cancer (2012)230507833.3300WBMarine pollution bulletin (2015)265635462.9910WB

規(guī)格價(jià)格50ul/780元 購(gòu)買    100ul/1280元 購(gòu)買    200ul/1980元 購(gòu)買    大包裝/詢價(jià)

說(shuō) 明 書(shū)50ul  100ul  200ul

研究領(lǐng)域腫瘤  細(xì)胞生物  染色質(zhì)和核信號(hào)  細(xì)胞凋亡  細(xì)胞周期蛋白  

抗體來(lái)源Rabbit

克隆類型Polyclonal

交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep,

P53 protein抗體產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test  ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量177kDa

細(xì)胞定位細(xì)胞漿

性    狀Lyophilized or Liquid

濃    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Nestin

亞    型IgG

純化方法affinity purified by Protein A

儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

P53 protein抗體產(chǎn)品介紹background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

P53 protein抗體溶解方法：

1、在我們公司購(gòu)買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml

2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無(wú)需再溶解）：

溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；

若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。

溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）

的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。

3、抗體的分裝

抗體溶解后，將其分裝成 5-10支（以0.1mlP53 protein抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。

另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。

后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。

我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。

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