ERK1 + ERK2抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術支持幫您更快得到實驗結果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質保服務,中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0022R
英文名稱ERK1 + ERK2
中文名稱絲裂原活化蛋白激酶1/ERK 1/2抗體
別 名ERK 1/2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; MK03_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.
JournalPMIDIFApplicationDrug Design, Development and Therapy (2015)266740843.0280WB
規(guī)格價格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書50ul 100ul 200ul
研究領域腫瘤 細胞生物 **學 神經(jīng)生物學 信號轉導 干細胞 細胞凋亡 轉錄調節(jié)因子 激酶和磷酸酶 細胞骨架
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
ERK1 + ERK2抗體產(chǎn)品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
ERK1 + ERK2抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
ERK1 + ERK2抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlERK1 + ERK2抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
ERK1 + ERK2抗體關聯(lián)產(chǎn)品列表：
bs-0166R YES 合格 合格 Neurokin B receptor 神經(jīng)激肽B受體抗體
bs-0169R YES 合格 Caspase-1 P10 天冬氨酸-胱氨酸特異性蛋白酶家族抗體
bs-0170R YES 合格 VEGFR1 血管內(nèi)皮生長因子受體1抗體
bs-0172R YES 合格 IRS1 胰島素受體底物-1抗體
bs-0173R YES 合格 IRS-2 胰島素受體底物-2抗體
bs-0175R YES 合格 TrkB 酪氨酸激酶B抗體
bs-0177R YES 合格 RAGE 晚期糖基化終末產(chǎn)物特異性受體抗體
bs-0178R No 合格 合格 Nurr1 核受體相關因子-1抗體
bs-0179R YES 合格 PSD95 突觸后密度蛋白95抗體
bs-0180R YES 合格 DOPA Decarboxylase 多巴胺脫羧酶抗體
bs-0182R No 合格 合格 PTP1B 蛋白酪氨酸磷酸酶-1B抗體
bs-0185R YES 合格 合格 PDGF-B 血小板源性生長因子-B抗體
bs-0188R No 合格 合格 Big endothelin-1 內(nèi)皮素-1抗體
bs-0189R YES 合格 合格 alpha smooth muscle Actin 肌動蛋白α/α-SMA/α Actin抗體
bs-0193R YES 合格 合格 合格 TrkA 酪氨酸激酶A抗體
bs-0195R YES 合格 CD31 血小板內(nèi)皮細胞黏附分子1抗體
bs-0196R YES 合格 PDGF-A 血小板源性生長因子A抗體
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