mTOR Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1648
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mTOR Rabbit
Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IHC
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H, M, R
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289.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實(shí)際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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-
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1:50-1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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mTOR Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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2475(Human);
56717(Mouse); 56718(Rat)
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SwissProt
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P42345(Human);
Q9JLN9(Mouse); P42346(Rat)
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Synonyms
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FRAP; FRAP1;
FRAP2; RXYT1; RAPT1
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Category
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PI3K/Akt/mTOR
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Background
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The
phosphatidylinositol kinase (PIK) family members fall into two distinct
subgroups. The first subgroup contains proteins such as the PI 3- and PI
4-kinases and the second group comprises the PIK-related kinases. The
PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in
common a region of homology at their carboxy-termini that is not present in
the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal
recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar
degeneration (ataxia) and the appearance of dilated blood vessels
(telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive
to ionizing radiation, impaired in mediating the inhibition of DNA synthesis
and display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding
enzyme that is composed of a large subunit, DNA-PKCS, and two smaller
subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB
repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind
to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has
been implicated in the regulation of p27 and p21 expression.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1648
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mTOR Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
mTOR Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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