CD31 Rabbit Monoclonal Antibody
|
產(chǎn)品編號(hào) |
產(chǎn)品名稱(chēng) |
產(chǎn)品包裝 |
產(chǎn)品價(jià)格 |
|
XY1642 |
CD31 Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來(lái)源 |
用途 |
交叉反應(yīng)性 |
分子量 |
|
Rabbit |
WB, IP, IHC, FC |
H |
130.0kDa |
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液,可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整):
|
WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
|
1:1,000-1:2,000 |
1:10-1:50 |
- |
1:50-1:200 |
- |
1:50-1:100 |
- |
- |
抗體詳細(xì)信息如下:
|
About this Antibody |
|
|
Name |
CD31 Rabbit Monoclonal Antibody |
|
Category |
Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
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Isotype |
IgG |
|
Purification |
XYfinity purification |
|
About the Immunogen |
|
|
Immunogen |
Recombinant protein |
|
Gene ID |
5175(Human) |
|
SwissProt |
P16284(Human) |
|
Synonyms |
CD31; CD31/EndoCAM; GPIIA'; PECA1; PECAM-1; endoCAM |
|
Category |
Immunology/Inflamation |
|
Background |
Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms' tumors, Ewing's sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the β2/α1 and β2/αM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism. |
包裝清單:
|
產(chǎn)品編號(hào) |
產(chǎn)品名稱(chēng) |
包裝 |
|
XY1642 |
CD31 Rabbit Monoclonal Antibody |
50μl |
|
XY050 |
Western一抗稀釋液 |
50ml |
|
- |
說(shuō)明書(shū) |
1份 |
保存條件:
CD31 Rabbit
Monoclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長(zhǎng)期不使用可以考慮-20℃保存,但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn),請(qǐng)注意回收使用過(guò)的稀釋抗體?;厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過(guò)的稀釋抗體,請(qǐng)4℃保存。
回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況,則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果,請(qǐng)考慮使用上述檢測(cè)專(zhuān)用的一抗稀釋液。
本產(chǎn)品**于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
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