5T4 Rabbit Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價格
|
|
XY1624
|
5T4 Rabbit
Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IP
|
H, M, R
|
72.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
1:10-1:50
|
-
|
-
|
-
|
-
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this
Antibody
|
|
Name
|
5T4 Rabbit
Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
7162(Human);
21983(Mouse); 83684(Rat)
|
|
SwissProt
|
Q13641(Human);
Q9Z0L0(Mouse); Q5PQV5(Rat)
|
|
Synonyms
|
Trophoblast
glycoprotein; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein;
5T4 oncotrophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1;
WAIF1; TPBG
|
|
Category
|
Stem Cells
& Wnt
|
|
Background
|
TPBG
(trophoblast glycoprotein), also known as 5T4, M6P1 or 5T4AG, is a 420 amino
acid single-pass type I membrane protein expressed by all types of
trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal
putative signal sequence, a 310-residue extracellular region, a membrane
anchorage domain and a 44-amino acid cytoplasmic tail with a potential
phosphorylation site. The extracellular region has seven potential
N-glycosylation sites and seven leucine-rich repeats, which are located in two
regions separated by a hydrophilic stretch. Suggested to be involved in cell
adhesion, TPBG may also be associated with tumor growth and progression.
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY1624
|
5T4 Rabbit
Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
5T4 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
- 溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買前務(wù)必確認(rèn)供應(yīng)商資質(zhì)與產(chǎn)品質(zhì)量。
- 免責(zé)申明：以上內(nèi)容為注冊會員自行發(fā)布，若信息的真實性、合法性存在爭議，平臺將會監(jiān)督協(xié)助處理，歡迎舉報