Cytokeratin 8+18 Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1618
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Cytokeratin
8+18 Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M
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53.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Cytokeratin
8+18 Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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3875/3856(Human);
16668/16691(Mouse)
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SwissProt
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P05783/P05787(Human);
P05784/P11679(Mouse)
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Synonyms
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CARD2; CK-8;
CK8; CYK8; K2C8; K8; KO
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Category
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Cytoskeletal
Signaling
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Background
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Cytokeratins
comprise a diverse group of intermediate filament proteins (IFPs) that are
expressed as pairs in both keratinized and non-keratinized epithelial tissue.
Cytokeratins play a critical role in differentiation and tissue specialization
and function to maintain the overall structural integrity of epithelial
cells. They have been found to be useful markers of tissue differentiation,
which is directly applicable to the characterization of malignant tumors.
Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors.
The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but
not in stratified epithelial cells. Research indicates that squamous cell
carcinomas derived from stratified epithelia show abnormal expression of
Cytokeratin 8 and 18, although it is not known whether these proteins
contribute to the malignant phenotype of the cells. Expression of Cytokeratin
8 and 18 in oral squamous cell carcinomas is an independent prognostic marker
that indicates a poor prognosis. Cytokeratin 8 expression correlates with
malignancy in leukoplakia and carcinomas of the head and neck; it is
expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to
possess extracellular epitopes on tumor cells, which may represent valuable
targets for therapy.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1618
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Cytokeratin
8+18 Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Cytokeratin 8+18
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
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