PKC alpha Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1576
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PKC alpha
Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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75.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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PKC alpha
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5578(Human);
18750(Mouse); 24680(Rat)
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SwissProt
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P17252(Human);
P20444(Mouse); P05696(Rat)
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Synonyms
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AAG6;
PKC-alpha; PKCA; PRKACA
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Category
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Cytoskeletal
Signaling; TGF-beta/Smad
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Background
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Members of the
protein kinase C (PKC) family play a key regulatory role in a variety of
cellular functions including cell growth and differentiation, gene
expression, hormone secretion and membrane function. PKCs were originally
identified as serine/threonine protein kinases whose activity was dependent
on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting
phorbol esters bind to and activate PKC. PKCs can be subdivided into many
different isoforms. Patterns of expression for each PKC isoform differ among
tissues and PKC family members exhibit clear differences in their cofactor
dependencies. For instance, the kinase activities of PKC ? and é are independent of Ca2+. On the other hand, most of the other PKC
members possess phorbol ester-binding activities and kinase activities.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1576
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PKC alpha
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
PKC alpha Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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