FUBP1 Rabbit Monoclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY1354
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FUBP1 Rabbit
Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IF, IHC,
ICC, FC
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H, M, R
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74.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液,可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整):
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:100-1:500
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1:50-1:200
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1:100-1:500
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1:50-1:100
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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FUBP1 Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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8880(Human);
51886(Mouse); 654496(Rat)
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SwissProt
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Q96AE4(Human);
Q91WJ8(Mouse); Q32PX7(Rat)
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Synonyms
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D3Ertd330e;
FUSE-binding protein 1; Far upstream element-binding protein 1
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Category
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Epigenetics
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Background
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Activation of
FUSE, the far upstream element, is required for the proper ex-pression of the
mammalian gene c-Myc in undifferentiated cells. The binding of FBP1
(FUSE-binding protein or far upstream element-binding protein) to FUSE is
necessary for c-Myc expression, indicating that FBP1 functions as a
growth-dependent regulator of c-Myc expression. Isolated from proliferating
HL-60 cells, FBP1 (FBP), FBP2 and FBP3 comprise a family of single-stranded
DNA-binding proteins that specifically bind to FUSE elements. The FBP
transcription factors share a conserved central DNA-binding domain and show
significant homology in their carboxyl-terminal activation domains.
Expression of FBP1 is detected in undifferentiated cells and is substantially
decreased following cellular differentiation.
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY1354
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FUBP1 Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
FUBP1 Rabbit
Monoclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長期不使用可以考慮-20℃保存,但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗,請注意回收使用過的稀釋抗體?;厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體,包括已經使用過的稀釋抗體,請4℃保存。
回收后重復使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況,則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果,請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業(yè)人員的科學研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內。
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