Recombinant Murine IL-5
產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY5921-1mg
1mg
15444.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
16191
P04401
E. coli
113aa
26.2kDa
－
About this Antibody
Name
Recombinant Murine IL-5 (Recombinant Murine Interleukin-5; rMuIL-5)；Recombinant Mouse IL-5 ; 重組小鼠白細(xì)胞介素5
Synonyms
BCDF mu; BCGFII; EDF; Eo-CSF; TRF; B-cell differentiation factor I; EDF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRFB cell differentiation factor I
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, pH9.0, 150mM NaCl.
Endotoxin
Less than 0.1EU/μg of rMuIL-5 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. The cDNA for murine IL-5 encodes a signal peptide and a 113 amino acid mature protein. Mature murine IL-5 shares 70%, 94%, 58%, 66%, 59% and 63%a.a. sequence identity with human, rat, canine, equine, feline and porcine IL5, respectively. It shows cross-reactivity with human IL5 receptor.
Amino Acid Sequence
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
包裝清單:
包裝
XY5921-5μg
5μg
XY5921-25μg
25μg
XY5921-100μg
100μg
-
說(shuō)明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。