- 如果您對(duì)該產(chǎn)品感興趣的話，可以
- 產(chǎn)品名稱：Recombinant Human TNF-α/TNFSF2, His-tag
- 產(chǎn)品型號(hào)：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無相關(guān)文檔
簡(jiǎn)單介紹
Recombinant Human TNF-α/TNFSF2, His-tag由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA???)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。Recombinant Human TNF-α/TNFSF2, His-tag所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
產(chǎn)品描述
Recombinant Human TNF-α/TNFSF2, His-tag
|
產(chǎn)品編號(hào)
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價(jià)格
|
|
XY322-1mg
|
Recombinant
Human TNF-α/TNFSF2, His-tag
|
1mg
|
9237.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
7124
|
P01375
|
E. coli
|
164aa
|
18.3kDa
|
His
|
|
About this
protein
|
|
Name
|
Recombinant
Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis
Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag)；重組人腫瘤壞死因子α/TNFSF2，His標(biāo)簽
|
|
Synonyms
|
Cachetin; DIF;
TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF,
monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived;
TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor
(TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis
factor ligand superfamily member 2; tumor necrosis factor-alpha
|
|
Purity
|
>97% by
SDS-PAGE and HPLC analyses.
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by a
cytotoxicity assay using murine L929 cells is less than 0.05ng/ml,
corresponding to a specific activity of >2.0×107IU/mg
in the presence of actinomycin D.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.0.
|
|
Endotoxin
|
Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as
determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Tumor necrosis
factor alpha (TNF-α), also called cachectin, is the best-know member of
the TNF-family, which can cause cell death. This protein is produced by
neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells,
astrocytes endothelial cells, smooth muscle cells and some transformed cells.
TNF-α occurs as a secreted, soluble form and as a
membrane-anchored form, both of which are biologically active. The
naturally-occurring form of TNF-α is glycosylated,
but non-glycosylated recombinant TNF-α has comparable
biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and
cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the
presence of one or both of these receptor types.
|
|
Amino Acid
Sequence
|
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE
GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL
LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
|
包裝清單:
|
產(chǎn)品編號(hào)
|
產(chǎn)品名稱
|
包裝
|
|
XY322-10μg
|
Recombinant
Human TNF-α/TNFSF2, His-tag
|
10μg
|
|
XY322-50μg
|
Recombinant
Human TNF-α/TNFSF2, His-tag
|
50μg
|
|
XY322-100μg
|
Recombinant
Human TNF-α/TNFSF2, His-tag
|
100μg
|
|
XY322-1mg
|
Recombinant
Human TNF-α/TNFSF2, His-tag
|
1mg
|
|
-
|
說明書
|
1份
|
保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
- 溫馨提示：為規(guī)避購(gòu)買風(fēng)險(xiǎn)，建議您在購(gòu)買前務(wù)必確認(rèn)供應(yīng)商資質(zhì)與產(chǎn)品質(zhì)量。
- 免責(zé)申明：以上內(nèi)容為注冊(cè)會(huì)員自行發(fā)布，若信息的真實(shí)性、合法性存在爭(zhēng)議，平臺(tái)將會(huì)監(jiān)督協(xié)助處理，歡迎舉報(bào)