LOCUS Exported 3747 bp ds-DNA circular SYN 11-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pEU-E01-MCS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3747)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3747
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..19
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(834..850)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 858..874
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(882..912)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 927..948
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1236..1824)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1995..2855)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIRYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2856..2960)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 atttaggtga cactatagaa ctcacctatc tccccaacac ctaataacat tcaatcactc
61 tttccactaa ccacctatct acatcaccaa gatatcacta gttctcgagc tcggtacctg
121 tccgcggtcg cgacgtacgc gggcggccgc cataaattgg atccatatat agggcccggg
181 ttataattac ctcaggtcga cgtcccatgg ttttgtatag aatttacggc tagcgccgga
241 tgcgacgccg gtcgcgtctt atccggcctt cctatatcag gcggtgttta agacgccgcc
301 gcttcgccca aatccttatg ccggttcgac gactggacaa aatactgttt atcttcccag
361 cgcaggcagg ttaatgtacc accccagcag cagccggtat ccagcgcgta tataccttcc
421 ggcgtacctt tgccctccag cgatgcccag tgaccaaagg cgatgctgta ttcttcagcg
481 acagggccag gaatcgcaaa ccacggtttc agtggggcag gggcctcttc cggcgattct
541 tactagctag tatgcatagg tgctgaaata taaagtttgt gtttctaaaa cacacgtggt
601 acgtacgata acgtacagtg tttttccctc cacttaaatc gaagggtagt gtcttggagc
661 gcgcggagta aacatatatg gttcatatat gtccgtaggc acgtaaaaaa agcgagggat
721 tcgaattccc ccggaacccc cggttggggc ccacgcctcg atcgagcaaa aaaaaaaaaa
781 aagaaaaaaa aaaaaaaaaa aagctttccc gcggccagct tggcgtaatc atggtcatag
841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
1021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcact cactgactcg
1081 ctgcgctcgg tcgctcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
1921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
1981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
2041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
2101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
2161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
2221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
2281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
2341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
2401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
2461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
2521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagcgtatg
2581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
2641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
2701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
2761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
2821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
2881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
2941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
3001 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
3061 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
3121 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
3181 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
3241 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
3301 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
3361 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
3421 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
3481 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
3541 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
3601 tccaaccaaa ccgactctgg cggcagttta cgagagagat gatagggtct gcttcagtaa
3661 gccagatgct acacaattag gcttgtacat actgtcgtta gaacgcggct acaattaata
3721 cataacctta tgtatcatac acatacg
//