LOCUS Exported 5992 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBARGPE1-Hygro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5992)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5992
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 18..34
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 81..97
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(147..831)
/note="gpdA Promoter"
misc_feature 1046..1186
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1372..1960)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2131..2991)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2992..3096)
/gene="bla"
/note="AmpR promoter"
promoter 3202..3303
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
misc_feature 3394..3779
/note="TrpC Promotor"
CDS 3780..4805
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
protein_bind 5197..5230
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 5308..5341
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5735..5965
/note="trpC terminator"
ORIGIN
1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
61 cccgggggat cctctagagt cgaggtcgac ggtatcgata agcttgatta aaggttcttg
121 gatgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
181 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
241 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
301 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
361 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
421 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
481 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
541 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
601 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
661 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
721 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggcgcagt
781 ggcgcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg ctttgcatac
841 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag
901 atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
1021 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
1081 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
1201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
1261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
2101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
2161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
2221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
2281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
2341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
2401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
2461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
2521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
2581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
2641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
2701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
2761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
2821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
2881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
2941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
3061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
3121 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
3181 gccctttcgt cttcaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
3241 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
3301 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
3361 cagagcacag aggccgcaga atgtgctcta gactcgacag aagatgatat tgaaggagca
3421 ctttttgggc ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc
3481 gtccaggcgg tgagcacaaa atttgtgtcg tttgacaaga tggttcattt aggcaactgg
3541 tcagatcagc cccacttgta gcagtagcgg cggcgctcga agtgtgactc ttattagcag
3601 acaggaacga ggacattatt atcatctgct gcttggtgca cgataacttg gtgcgtttgt
3661 caagcaaggt aagtgaacga cccggtcata ccttcttaag ttcgcccttc ctccctttat
3721 ttcagattca atctgactta cctattctac ccaagcaaag cttcgattag gaagtaacca
3781 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
3841 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
3901 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
3961 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
4021 gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
4081 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
4141 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
4201 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
4261 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
4321 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
4381 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
4441 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
4501 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
4561 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
4621 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
4681 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
4741 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga
4801 aatagtccgt cgacctgcag atcgttcaaa catttggcaa taaagtttct taagattgaa
4861 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
4921 aataattaac atgtaatgca tagtaccgag aaactagtgg atctaaacat aaaatctgta
4981 aaataacaag atgtaaagat aatgctaaat catttggctt tttgattgat tgtacaggaa
5041 aatatacatc gcagggggtt gacttttacc atttcaccgc aatggaatca aacttgttga
5101 agagaatgtt cacaggcgca tacgctacaa tgacccgatt cttgctagcc ttttctcggt
5161 cttgcaaaca accgccgcgg ccggtcgagg gacctaataa cttcgtatag catacattat
5221 acgaagttat attaagggtt ccggatcgcg gccgctcgac ctgcagccaa gctagcttgg
5281 ctggacgtaa actcctcttc agacctaata acttcgtata gcatacatta tacgaagtta
5341 tattaagggt tattgaatat gatcggaatt ggtcgatcag gtattgctgt tatctgatga
5401 gtatacgttg tcctggccac ggcagaagca cgcttatcgc tccaatttcc cacaacatta
5461 gtcaactccg ttaggccctt cattgaaaga aatgaggtca tcaaatgtct tccaatgtga
5521 gattttgggc cattttttat agcaaagatt gaataaggcg catttttctt caaagcttgc
5581 aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac
5641 atgcgtacac gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
5701 taatcataaa aacccatctc ataaataacg tcatgcattg cagatgagct gtatctggaa
5761 gaggtaaacc cgaaacgcgt tttattcttg ttgacatgga gctattaaat cactagaagg
5821 cactctttgc tgcttggaca aatgaacgta tcttatcgag atcctgaaca ccatttgtct
5881 caactccgga gctgacatcg acaccaacga tcttatatcc agattcgtca agctgtttga
5941 tgatttcagt aacgttaagt ggatctcggt gacgggcagg accggacggg gc
//