LOCUS Exported 7055 bp ds-DNA circular SYN 08-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..7055
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(311..1171)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1172..1276)
/gene="bla"
/label=AmpR promoter
CDS 3394..4209
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
protein_bind 4718..4734
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
rep_origin 4769..5157
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(5775..6146)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 6179..6288
/note="incP origin of transfer"
ORIGIN
1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
2881 atcagatctg gccgcctagg ccgaattcga gctcggtacc cggggatccg gctgtaatcc
2941 gggcagcgca acggaacatt catcagtgta aaaatggaat caataaagcc ctgcgcagcg
3001 cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc aaggtcagaa
3061 tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc
3121 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
3181 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
3241 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct
3301 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
3361 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
3421 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
3481 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
3541 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
3601 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
3661 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
3721 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
3781 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
3841 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
3901 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
3961 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
4021 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
4081 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
4141 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
4201 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
4261 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
4321 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
4381 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
4441 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctatt
4501 ctgaccttgc catcacgact gtgctggtca ttaaacgcgt attcaggctg accctgcgcg
4561 ctgcgcaggg ctttattgat tccattttta cactgatgaa tgttccgttg cgctgcccgg
4621 attacagccg gatcctctag agtcgacctg caggcatgca agcttcggcc gcctaggccg
4681 cggccgcact tgtgtataag agtcagaatt ccacaaattg ttatccgctc acaattccac
4741 atgtggaatt ccacatgtgg aattcccatg tcagccgtta agtgttcctg tgtcactcaa
4801 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
4861 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
4921 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
4981 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
5041 atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg
5101 tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct tcagatcctc
5161 tacgccggac gcatcgtggc cggatccagc cgaccaggct ttccacgccc gcgtgccgct
5221 ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg tcacgctcaa
5281 gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag gcccgatacg
5341 gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg atgtgcaggt
5401 tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca agcccagcgc
5461 agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc gcgcggaagc
5521 taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc tgggtcgcca
5581 tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg cgcacgtgac
5641 ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc
5701 tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt
5761 agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca
5821 agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac
5881 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg
5941 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg
6001 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt
6061 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc
6121 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
6181 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
6241 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
6301 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
6361 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
6421 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg
6481 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg
6541 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg
6601 ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc
6661 atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac gttccgctcg
6721 cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca ggccaaggct
6781 gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca ggggggcaag
6841 gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc aacaccggac
6901 aaaaaggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
6961 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
7021 catgagcgct tgtttcggcg tgggtatggt ggcag
//