如果您對(duì)該產(chǎn)品感興趣的話，可以
產(chǎn)品名稱：pBAD/Myc-HisC
產(chǎn)品型號(hào)：
產(chǎn)品展商：XYbscience
產(chǎn)品文檔：無(wú)相關(guān)文檔

簡(jiǎn)單介紹

pBAD/Myc-HisC菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證，如存在質(zhì)量問(wèn)題，請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。pBAD/Myc-HisC收到質(zhì)粒后請(qǐng)短暫離心，加入20μl ddH2O溶解質(zhì)粒，取2μl轉(zhuǎn)化至對(duì)應(yīng)感???態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。

產(chǎn)品描述

pBAD/Myc-HisC

產(chǎn)品信息
基本信息
質(zhì)粒簡(jiǎn)介
質(zhì)粒圖譜
質(zhì)粒序列

產(chǎn)品信息

產(chǎn)品貨號(hào)	產(chǎn)品名稱	產(chǎn)品規(guī)格	優(yōu)惠價(jià)
XY0803	pBAD/Myc-HisC	20μl	￥請(qǐng)?jiān)儍r(jià)

使用說(shuō)明

信裕質(zhì)粒平臺(tái)的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證，如存在質(zhì)量問(wèn)題，請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到質(zhì)粒后請(qǐng)短暫離心，加入20μl ddH2O溶解質(zhì)粒，取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。如需獲取其他詳細(xì)信息請(qǐng)登錄我司官網(wǎng)查詢。

基本信息

啟動(dòng)子:	araBad
復(fù)制子:	PBR322 ori
質(zhì)粒分類:	大腸桿菌載體；pBAD系列表達(dá)質(zhì)粒
質(zhì)粒大小:	4093bp
質(zhì)粒標(biāo)簽:	C-His,C-Myc Epitope
原核抗性:	氨芐青霉素Amp
克隆菌株:	Top10
培養(yǎng)條件:	37℃，有氧，LB
表達(dá)宿主:	推薦LMG194
培養(yǎng)條件:	37℃，有氧，LB
誘導(dǎo)方式:	阿拉伯糖
5'測(cè)序引物:	pBAD-F: ATGCCATAGCATTTTTATCC
3'測(cè)序引物:	pBAD-R: GATTTAATCTGTATCAGG
備注:	pBAD/Myc-His C 載體是阿拉伯糖調(diào)控載體；在無(wú)葡萄糖的培養(yǎng)基中，阿拉伯糖正向調(diào)控目的基因的表達(dá)；通過(guò)調(diào)節(jié)阿拉伯糖的濃度水平來(lái)優(yōu)化目的蛋白的可溶性表達(dá)

質(zhì)粒簡(jiǎn)介

pBAD/His和PBAD/Myc-His載體質(zhì)粒是衍生于pBR322載體。載體設(shè)計(jì)用來(lái)在大腸桿菌(Escherichia coli)中進(jìn)行可調(diào)節(jié)，劑量依賴性的表達(dá)和純化重組目的蛋白。使用大腸桿菌(Escherichia coli)araBAD啟動(dòng)子（pBAD）增強(qiáng)了大腸桿菌(Escherichia coli)重組蛋白可溶性表達(dá)的水平。pBAD/His和pBAD/Myc His載體上的調(diào)節(jié)蛋白AraC能夠調(diào)控pBad啟動(dòng)子。

質(zhì)粒圖譜

質(zhì)粒序列

LOCUS       Exported                4093 bp ds-DNA     circular SYN 23-九月-2016
DEFINITION synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 2
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 4093)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported 2016年9月23日 from SnapGene Viewer 2.8.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..285
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             376..405
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             421..438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      664..750
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      842..869
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        888..979
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             980..1840
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2011..2599
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2785..2925
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3189..4067)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa
      361 ttcgaagctt acgtagaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac
      421 catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt ggcggatgag
      481 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
      541 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      601 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      661 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      721 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      781 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
      841 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
      901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      961 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1021 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1081 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1141 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1201 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     1261 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1321 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1381 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1441 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1501 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1561 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1621 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1681 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1741 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1801 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1861 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1921 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2761 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2821 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2881 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     2941 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3001 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3061 aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg
     3121 gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc
     3181 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg
     3241 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg
     3301 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc
     3361 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg
     3421 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata
     3481 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta
     3541 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca
     3601 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt
     3661 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta
     3721 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa
     3781 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct
     3841 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc
     3901 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg
     3961 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca
     4021 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc
     4081 ccgccattca gag
//

產(chǎn)品留言

標(biāo)題

聯(lián)系人

聯(lián)系電話

內(nèi)容

驗(yàn)證碼

點(diǎn)擊換一張

注：1.可以使用快捷鍵Alt+S或Ctrl+Enter發(fā)送信息!
2.如有必要,請(qǐng)您留下您的詳細(xì)聯(lián)系方式!

溫馨提示：為規(guī)避購(gòu)買風(fēng)險(xiǎn)，建議您在購(gòu)買前務(wù)必確認(rèn)供應(yīng)商資質(zhì)與產(chǎn)品質(zhì)量。
免責(zé)申明：以上內(nèi)容為注冊(cè)會(huì)員自行發(fā)布，若信息的真實(shí)性、合法性存在爭(zhēng)議，平臺(tái)將會(huì)監(jiān)督協(xié)助處理，歡迎舉報(bào)

国产精品久久久久久亚洲,国产成人无码午夜视频在线观看 ,国产福利一区二区三区在线观看,国产av第一次处破,厨房玩弄丝袜人妻系列国产电影

產(chǎn)品目錄

產(chǎn)品資料

pBAD/Myc-HisC

pBAD/Myc-HisC

產(chǎn)品信息

使用說(shuō)明

基本信息

質(zhì)粒簡(jiǎn)介

質(zhì)粒圖譜

質(zhì)粒序列