質(zhì)粒序列
LOCUS Exported 3438 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing untagged proteins. For other reading
frames, use pQE-51 or pQE-52.
ACCESSION .
VERSION .
KEYWORDS pQE-50
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3438)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3438
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
misc_feature 121..168
/note="MCS"
/note="multiple cloning site"
misc_feature 168..178
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 184..278
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 322..981
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1046..1132
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1614..2202)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2373..3233)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3234..3338)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatccgcat gcgagctcgg taccccgggt cgacctgcag ccaagcttaa ttagctgagc
181 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
241 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagctt ggcgagattt
301 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
361 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
421 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
481 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt
541 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
601 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
661 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
721 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
781 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
841 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
901 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
961 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggg
1021 gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
1081 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgct
1141 ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
1201 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
1261 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
1321 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
1381 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
1441 tcctcgctca ctgactcgct gcgctcggtc tgtcggctgc ggcgagcggt atcagctcac
1501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
1561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
1621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
1681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
1741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
1801 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
1861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
1921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
1981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
2041 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
2101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
2161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
2221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
2281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
2341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
2401 atctcagcga tctgtctatt tcgttcatcc atagctgcct gactccccgt cgtgtagata
2461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
2521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
2581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
2641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
2701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
2761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
2821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
2881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
2941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
3001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
3061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
3121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
3181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
3241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
3301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
3361 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
3421 aggccctttc gtcttcac
//