質(zhì)粒序列
LOCUS Exported 276 bp ds-DNA linear SYN 10-SEP-2012
DEFINITION Cloning region of pQE-T7, the parental vector for the QIAgenes
system.
ACCESSION .
VERSION .
KEYWORDS pQE-T7 (cloning region)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 276)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT The 6xHis tag can be removed by cutting with NdeI, purifying the cut
vector, and religating.
The QIAgene can be excised with NdeI and XhoI.
FEATURES Location/Qualifiers
source 1..276
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 16..34
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 35..59
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 104..106
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 110..127
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 128..130
/codon_start=1
/note="QIAgene start codon"
/translation="M"
misc_feature 131..136
/note="stop codons"
/note="two tandem stop codons"
CDS 145..162
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 229..276
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatgaaac accatcacca
121 tcaccatatg tagtaagact cgagcaccac caccaccacc actgagatcc ggctgctaac
181 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
241 cttggggcct ctaaacgggt cttgaggggt tttttg
//