質(zhì)粒序列
LOCUS Exported 5796 bp ds-DNA circular SYN 05-MAY-2014
DEFINITION Vector for expression in bacterial, insect, and vertebrate cells of
proteins with an N-terminal Strep-Tag II tag and a C-terminal 8xHis
tag.
ACCESSION .
VERSION .
KEYWORDS pQE-TriSystem His-Strep2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5796)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5796
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 62..367
/note="CMV enhancer"
/note="human cytomegalovirus (CMV) immediate early
enhancer"
promoter 373..650
/note="chicken beta-actin promoter"
exon 651..742
/note="Exon 1"
intron 743..1004
exon 1005..1649
/note="Exon 2"
promoter 1073..1116
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 1092..1108
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 1143..1252
/note="p10 promoter"
/note="baculovirus promoter for expression in insect cells"
RBS 1255..1260
/note="ribosome binding site"
CDS 1267..1269
/codon_start=1
/note="ATG"
/note="start codon"
/translation="M"
CDS 1279..1302
/codon_start=1
/product="peptide that binds Strep-Tactin, an engineered
form of streptavidin"
/note="Strep-Tag II"
/translation="WSHPQFEK"
misc_feature 1307..1446
/note="MCS"
/note="multiple cloning site"
CDS 1408..1440
/codon_start=1
/product="HSV (herpes simplex virus) epitope tag"
/note="HSV tag"
/translation="QPELAPEDPED"
CDS 1447..1470
/codon_start=1
/product="8xHis affinity tag"
/note="8xHis"
/translation="HHHHHHHH"
misc_feature 1471..1481
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 1625..1630
terminator 1647..1758
/note="rabbit globin terminator"
terminator 1768..1815
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_recomb 1828..3286
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(1945..3286)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(3445..4032)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4045..4905)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_recomb 4971..2
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 4971..5095
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(5133..5738)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
1261 tataccatgg cgagcgcgtg gagccacccg cagttcgaaa aaatatcccg ggagctcgtg
1321 gatccgaatt ctcagatctc ggcgcgcctg caggtcgacg gtaccggttc gaagcttgcg
1381 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
1441 ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct
1501 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
1561 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
1621 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
1681 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagtt tttggtttag
1741 agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc
1801 ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt
1861 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
1921 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
1981 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
2041 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
2101 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
2161 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
2221 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
2281 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
2341 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
2401 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
2461 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
2521 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
2581 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
2641 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
2701 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
2761 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
2821 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
2881 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
2941 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
3001 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
3061 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
3121 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
3181 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
3241 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcggaacgc tgcgctcggt
3301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
3421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4021 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
4081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
4141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
4201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
4261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
4321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
4381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
4441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
4501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
4561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
4621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
4681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
4741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
4801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
4861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
4921 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtttcc tgcatctttt
4981 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
5041 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
5101 aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
5161 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
5221 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
5281 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
5341 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
5401 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
5461 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
5521 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
5581 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
5641 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
5701 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
5761 tatcacaaac tggaaatgtc tatcaatata tagttg
//