質(zhì)粒序列
LOCUS Exported 5292 bp ds-DNA circular SYN 05-MAY-2014
DEFINITION Vector for expressing N-terminally 10xHis-tagged proteins in insect
and mammalian cells using the QIAgenes system.
ACCESSION .
VERSION .
KEYWORDS pQE-Trisystem-5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5292)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5292
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_recomb 181..1008
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 181..305
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(343..948)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
enhancer 1138..1443
/note="CMV enhancer"
/note="human cytomegalovirus (CMV) immediate early
enhancer"
promoter 1449..1726
/note="chicken beta-actin promoter"
exon 1727..1819
/note="Exon 1"
intron 1820..2081
exon 2082..2676
/note="Exon 2"
promoter 2150..2168
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 2169..2193
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 2209..2318
/note="p10 promoter"
/note="baculovirus promoter for expression in insect cells"
RBS 2321..2326
/note="ribosome binding site"
CDS 2333..2335
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 2339..2368
/codon_start=1
/product="10xHis affinity tag"
/note="10xHis"
/note="can be processed with TAGZyme"
/translation="HHHHHHHHHH"
misc_feature 2368..2446
/note="MCS"
/note="multiple cloning site"
misc_feature 2449..2467
/note="siRNA target site"
CDS 2474..2497
/codon_start=1
/product="8xHis affinity tag"
/note="8xHis"
/translation="HHHHHHHH"
misc_feature 2498..2508
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 2652..2657
terminator 2674..2785
/note="rabbit globin terminator"
terminator 2795..2842
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_feature 2855..3560
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2972..3560)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(3770..4357)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4370..5230)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaagca
2341 ccaccaccat caccatcatc accaccatca gtgaagagcg atatcccggg tggatccgaa
2401 ttctcagatc tcggcgcgcc tgcagggttc gaagcttata ctcgagaagc gttgaaatag
2461 cgtacaagtc gagcaccacc atcaccatca ccatcactaa gtgattaacc tcaggtgcag
2521 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
2581 gatcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
2641 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
2701 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
2761 ggtttagagt ttggcaacat atgcccatat gtaactagca taaccccttg gggcctctaa
2821 acgggtcttg aggggttttt tgctgaaagc atgcggagga aattctcctt gaagtttccc
2881 tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt ccggcgtatt
2941 aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg cgttgttgat
3001 ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt
3061 atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaaat
3121 cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt ttttccgaac
3181 cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc aaatcttgta
3241 gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt
3301 cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg tacaattgac
3361 tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg ttagctttat
3421 taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc gaagacgatt
3481 ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg atcaaatttg
3541 tagttgagct ttttggaatt gcgatcgcat aacttcgtat agcatacatt atacgaagtt
3601 ataagctcgg aacgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
3781 tccgccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
3841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3961 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
4021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
4261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
4321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttgt taccaatgct
4381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
4921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
4981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5281 tgtccgcgcg tt
//