質(zhì)粒序列
LOCUS Exported 2887 bp ds-DNA circular SYN 11-9-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2887)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-9-11 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2887
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
terminator 302..349
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 446..901
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 927..1031
/gene="bla"
/note="AmpR promoter"
CDS 1032..1892
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2063..2651
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatccgag ctcgagatct gcagctggta ccatggaatt cgaagcttga
241 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
301 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
361 aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
421 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
661 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
781 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
841 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
901 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
961 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
1021 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
1081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
1141 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
1201 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
1261 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
1321 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
1381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
1441 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
1501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
1561 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
1621 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
1681 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
1741 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
1801 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
1861 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1921 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1981 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
2041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
2101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
2161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
2221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
2281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
2341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
2401 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
2461 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
2521 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
2581 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
2641 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
2701 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
2761 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
2821 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
2881 aatgcag
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