質(zhì)粒序列
LOCUS Exported 2895 bp ds-DNA circular SYN 11-9-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2895)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-9-11 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2895
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
terminator 310..357
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 454..909
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 935..1039
/gene="bla"
/note="AmpR promoter"
CDS 1040..1900
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2071..2659
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatcgatg gatccgacct cgagatctgc agctggtacc atggaattcg
241 aagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
301 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
361 aaaggaggaa ctatatccgg atctggcgta atagcgaaga ggcccgcacc gatcgccctt
421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
901 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
1021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
1081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
1141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
1201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
1261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
1321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
1381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
1441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
1501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
1561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
1621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
1681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
1741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
1801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
1861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
1921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
1981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
2041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
2101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
2221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
2641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
2701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
2761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
2821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
2881 gattcattaa tgcag
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