質(zhì)粒序列
LOCUS Exported 3437 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing untagged proteins. For other reading
frames, use pQE-50 or pQE-52.
ACCESSION .
VERSION .
KEYWORDS pQE-51
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3437)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3437
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
misc_feature 120..167
/note="MCS"
/note="multiple cloning site"
misc_feature 167..177
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 183..277
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 321..980
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1045..1131
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1613..2201)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2372..3232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3233..3337)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
121 gatccgcatg cgagctcggt accccgggtc gacctgcagc caagcttaat tagctgagct
181 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
241 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt
301 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
361 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
421 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
481 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
541 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
601 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
661 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
721 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
781 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
841 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
901 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
961 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg
1021 taatgactct ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
1081 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgctc
1141 tagagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
1201 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
1261 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
1321 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
1381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
1441 cctcgctcac tgactcgctg cgctcggtct gtcggctgcg gcgagcggta tcagctcact
1501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
1561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
1621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
1681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
1741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1801 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
1861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
1921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
1981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
2101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
2161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
2281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
2341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
2401 tctcagcgat ctgtctattt cgttcatcca tagctgcctg actccccgtc gtgtagataa
2461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
2521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
2581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
2641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
2701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
2761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
2821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
2881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
2941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
3121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
3181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
3241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
3301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
3361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
3421 ggccctttcg tcttcac
//