質(zhì)粒序列
LOCUS Exported 4033 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing DHFR fusion proteins with an
N-terminal 6xHis tag. For other reading frames, use pQE-40 or
pQE-41.
ACCESSION .
VERSION .
KEYWORDS pQE-42
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4033)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..4033
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 157..714
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/note="DHFR"
/translation="MVRPLNSIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKK"
misc_feature 722..763
/note="MCS"
/note="multiple cloning site"
misc_feature 763..773
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 779..873
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 917..1576
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1641..1727
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2209..2797)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2968..3828)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3829..3933)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttgg
721 aagatctgca tgcggtaccc cgggtcgacc tgcagccaag cttaattagc tgagcttgga
781 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
841 ggttgccgcc gggcgttttt tattggtgag aatccaagct agcttggcga gattttcagg
901 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
961 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
1021 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
1081 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
1141 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
1201 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
1261 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
1321 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
1381 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
1441 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
1501 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
1561 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat
1621 gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
1681 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgctctaga
1741 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
1801 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
1861 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
1921 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
1981 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
2041 gctcactgac tcgctgcgct cggtctgtcg gctgcggcga gcggtatcag ctcactcaaa
2101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
2161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
2221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
2281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
2341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
2401 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
2461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
2521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
2581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
2641 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
2701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
2761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
2821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
2881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
2941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
3001 agcgatctgt ctatttcgtt catccatagc tgcctgactc cccgtcgtgt agataactac
3061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
3121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
3181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
3241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
3301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
3361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
3421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
3481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
3541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
3601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
3661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
3721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
3781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
3841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
3901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
3961 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
4021 ctttcgtctt cac
//