質粒序列
LOCUS Exported 5677 bp ds-DNA circular SYN 15-JUN-2017
DEFINITION synthetic circular DNA.
KEYWORDS pET-11a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5677)
TITLE Direct Submission
JOURNAL Exported Tuesday, July 11, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5677
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..19
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 89..121
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
terminator 189..236
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 411..439
/note="tet promoter"
/note="promoter for tetracycline efflux protein gene"
promoter 552..656
/gene="bla"
/note="AmpR promoter"
CDS 657..1517
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1688..2276
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2706..2897)
/codon_start=1
/gene="rop"
/product="Rop protein"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(4209..5291)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 5292..5369
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
61 tgtttaactt taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg
121 tcgcggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
181 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
241 aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
301 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
361 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
421 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
1021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
1681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
1801 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
1861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
1921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
1981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
2041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
2101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
2221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
2281 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
2401 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2461 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
2521 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
2581 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
2641 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
2701 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
2761 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
2821 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
2881 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
2941 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
3001 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
3061 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
3121 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
3181 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
3241 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
3301 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
3361 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac
3421 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
3481 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
3541 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
3601 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
3661 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
3721 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
3781 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
3841 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
3901 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
3961 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
4021 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
4081 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
4141 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
4201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
4261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
4321 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
4381 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
4441 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
4501 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
4561 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
4621 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
4681 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
4741 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
4801 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
4861 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
4921 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
4981 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
5041 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
5101 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
5161 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
5221 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
5281 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
5341 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
5401 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
5461 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
5521 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
5581 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
5641 tccggcgtag aggatcgaga tctcgatccc gcgaaat
//