質(zhì)粒序列
LOCUS Exported 4639 bp ds-DNA circular SYN 28-7-2015
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4639)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-7-28 from MLCC
http://www.miaolingbio.com/
FEATURES Location/Qualifiers
source 1..4639
/organism="synthetic DNA construct"
/mol_type="other DNA"
source 558..580
/organism="Enterobacteria phage T7"
/mol_type="genomic DNA"
/db_xref="taxon:10760"
promoter 10..38
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator 404..451
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(518..550)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
RBS 558..580
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
promoter complement(612..630)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2191..2382
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 2484..2626
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(2812..3400)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3571..4431)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4432..4536)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatcccgacc catttgctgt ccaccagtca
541 tgctagccat atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc
601 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
661 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
721 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
781 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
841 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
901 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
961 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
1021 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
1081 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
1141 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
1201 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
1261 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
1321 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
1381 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
1441 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
1501 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
1561 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
1621 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
1681 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
1741 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
1801 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
1861 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
1921 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
1981 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
2041 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
2101 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
2161 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
2221 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
2281 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
2341 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
2401 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
2461 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
2521 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg
2581 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
2641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
2701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
2761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
3001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
3061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
3121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
3181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
3241 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
3301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
3361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
3421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
3481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
3541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
3601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
3661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
3721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
3781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
3841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
3901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
3961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
4021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
4081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
4141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
4201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
4261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
4321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
4381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
4441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
4501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
4561 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
4621 gccctttcgt cttcaagaa
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