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pBAD/His-B

pBAD/His-B
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簡單介紹
pBAD/His-B是衍生于pBR322載體。載體設(shè)計用來在大腸桿菌中進(jìn)行可調(diào)節(jié)，劑量依賴性的表達(dá)和純化重組目的蛋白。pBAD/His-B使大腸桿菌araBAD啟動子（pBAD）增強(qiáng)了大腸桿菌重組蛋白可溶性表達(dá)的水平。pBAD/His和pBAD/Myc His載體上的調(diào)節(jié)蛋白AraC能夠調(diào)控pBad啟動子。pBAD/Myc-His B 載體是阿拉伯糖調(diào)控載體，在無葡萄糖的培養(yǎng)基中，阿拉伯糖正向調(diào)控目的基因的表達(dá)，通過調(diào)節(jié)阿拉伯糖的濃度水平來優(yōu)化目的蛋白的可溶性表達(dá)。
產(chǎn)品描述
 

pBAD/His-B

  • 產(chǎn)品信息
  • 基本信息
  • 質(zhì)粒簡介
  • 質(zhì)粒圖譜
  • 質(zhì)粒序列

產(chǎn)品信息

產(chǎn)品貨號 產(chǎn)品名稱 產(chǎn)品規(guī)格 優(yōu)惠價
XY0080 pBAD/His-B

5μg

￥請詢價

使用說明

信裕質(zhì)粒平臺的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴(yán)格的多重驗證，如存在質(zhì)量問題，請在收到產(chǎn)品的三個月內(nèi)通知我司。收到質(zhì)粒后請短暫離心，加入20μl ddH2O溶解質(zhì)粒，取2μl轉(zhuǎn)化至對應(yīng)感受態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。如需獲取其他詳細(xì)信息請登錄我司官網(wǎng)查詢。

基本信息

啟動子: araBAD promoter
復(fù)制子: pBR322 ori
終止子: rrnB T1 terminator
質(zhì)粒分類: 大腸系列質(zhì)粒；大腸表達(dá)質(zhì)粒；pBAD系列質(zhì)粒
質(zhì)粒大小: 4092bp
質(zhì)粒標(biāo)簽: N-His, N-EK，N-xPress Epitope
原核抗性: 氨芐青霉素Amp（100μg/ml）
克隆菌株: Top10等大腸桿菌
培養(yǎng)條件: 37℃，有氧，LB
表達(dá)宿主: LMG194等大腸桿菌
培養(yǎng)條件: 37℃，有氧，LB
誘導(dǎo)方式: 阿拉伯糖
5'測序引物: PBAD30.F（AGATTAGCGGATCCTACCTG）
3'測序引物: PBAD30.R（CACTTCTGAGTTCGGCATGG）

質(zhì)粒簡介

        pBAD-HisB質(zhì)粒是衍生于pBR322載體。載體設(shè)計用來在大腸桿菌中進(jìn)行可調(diào)節(jié)，劑量依賴性的表達(dá)和純化重組目的蛋白。使大腸桿菌araBAD啟動子（pBAD）增強(qiáng)了大腸桿菌重組蛋白可溶性表達(dá)的水平。pBAD/His和pBAD/Myc His載體上的調(diào)節(jié)蛋白AraC能夠調(diào)控pBad啟動子。pBAD/Myc-His B 載體是阿拉伯糖調(diào)控載體，在無葡萄糖的培養(yǎng)基中，阿拉伯糖正向調(diào)控目的基因的表達(dá)，通過調(diào)節(jié)阿拉伯糖的濃度水平來優(yōu)化目的蛋白的可溶性表達(dá)。

質(zhì)粒圖譜



質(zhì)粒序列

LOCUS       Exported                4092 bp ds-DNA     circular SYN 22-JUN-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pBAD-HisB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, June 22, 2017 from MiaoLingPlasmid
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..285
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             331..348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             352..384
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             388..411
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /note="Xpress(TM) tag"
                     /translation="DLYDDDDK"
     misc_feature    420..460
                     /note="MCS"
     terminator      663..749
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      841..868
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        887..978
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             979..1839
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2010..2598
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2784..2924
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3188..4066)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
      361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
      421 tcgagatctg cagctggtac catatgggaa ttcgaagctt ggctgttttg gcggatgaga
      481 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
      541 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
      601 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
      661 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      721 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
      781 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
      841 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
      901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1201 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     1261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2761 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2821 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2881 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     2941 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3061 ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc tgtcaaatgg
     3121 acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg
     3181 ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
     3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
     3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
     3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
     3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
     3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
     3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
     3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
     3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
     3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
     3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
     3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
     3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
     3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
     4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
     4081 cgccattcag ag
//
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