質(zhì)粒序列
LOCUS Exported 3426 bp ds-DNA circular SYN 08-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS PQE-70
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3426)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3426
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 133..150
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 173..267
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 311..970
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1035..1121
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1276..1416
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1602..2190)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2361..3221)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3222..3326)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aagcatgcga
121 ggatccagat ctcatcacca tcaccatcac taagcttaat tagctgagct tggactcctg
181 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
241 cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa
301 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
361 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
421 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
481 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
541 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
601 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
661 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
721 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
781 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
841 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
901 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
961 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct
1021 ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
1081 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccctct agagctgcct
1141 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
1201 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
1261 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
1321 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
1381 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
1441 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
1501 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
1561 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
1621 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
1681 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
1741 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
1801 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
1861 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
1921 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
1981 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
2041 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
2101 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
2161 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
2221 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
2281 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
2341 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
2401 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
2461 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
2521 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
2581 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
2641 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
2701 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
2761 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
2821 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
2881 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
2941 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
3001 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
3061 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
3121 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
3181 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
3241 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
3301 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
3361 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
3421 cttcac
//