LOCUS Exported 4613 bp ds-DNA circular SYN 11-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4613)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4613
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1001..1285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 1306..1362
/label=MCS
/note="pUC18/19 multiple cloning site"
terminator 1565..1651
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1743..1770
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1789..1880
/gene="bla"
/label=AmpR promoter
CDS 1881..2741
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2783..3238
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 3349..3937
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4123..4263
/label=bom
/note="basis of mobility region from pBR322"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
2461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
2521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
2581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
2641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
2701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
2761 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
2821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
2881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
2941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
3001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
3061 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc
3121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
3181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
3241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
3301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
3601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
3661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
3721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
3781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
3841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
3901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
3961 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
4021 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
4081 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
4141 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
4201 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
4261 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
4321 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
4381 tcatcaccga aacgcgcgag gcagcaagga gatggcgccc aacagtcccc cggccacggg
4441 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
4501 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
4561 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc
//