質粒簡介
pBAD/His和PBAD/Myc-His載體質粒是衍生于pBR322載體。載體設計用來在大腸桿菌中進行可調節(jié)，劑量依賴性的表達和純化重組目的蛋白。使用大腸桿菌araBAD啟動子（pBAD）增強了大腸桿菌重組蛋白可溶性表達的水平。pBAD/His和pBAD/Myc His載體上的調節(jié)蛋白AraC能夠調控pBad啟動子。
質粒序列
LOCUS Exported 4094 bp ds-DNA circular SYN 11-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS pBAD Myc-HisA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4094)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4094
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 377..406
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 422..439
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 665..751
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 843..870
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 889..980
/gene="bla"
/note="AmpR promoter"
CDS 981..1841
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2012..2600
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2786..2926
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(3190..4068)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa
361 ttcgaagctt gggcccgaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga
421 ccatcatcat catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga
481 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
541 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
601 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
661 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
721 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
781 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
841 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
1141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
1201 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
1261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
1321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
1381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
1441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
1501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
1561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
1621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
1681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
1741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
2101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
2161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
2221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
2281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
2341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
2401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
2461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
2521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
2581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
2641 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
2701 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
2761 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
2821 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
2881 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
2941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
3001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
3061 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat
3121 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
3181 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
3241 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
3301 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
3361 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
3421 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
3481 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
3541 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
3601 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
3661 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
3721 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
3781 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
3841 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
3901 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
3961 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
4021 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
4081 cccgccattc agag
//