質粒序列
LOCUS Exported 4092 bp ds-DNA circular SYN 11-九月-2016
DEFINITION synthetic circular DNA
KEYWORDS pBAD Myc-HisB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4092)
TITLE Direct Submission
JOURNAL Exported 2016年9月11日 from SnapGene Viewer 2.8.0
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4092
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 375..404
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 420..437
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 663..749
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 841..868
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 887..978
/gene="bla"
/note="AmpR promoter"
CDS 979..1839
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2010..2598
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2784..2924
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(3188..4066)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa
361 ttcgaagctt tctagaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc
421 atcatcatca tcatcattga gtttaaacgg tctccagctt ggctgttttg gcggatgaga
481 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
541 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
601 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
661 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
721 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
781 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
841 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
1021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
1081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1201 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
1261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
1561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
1621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
1681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
1741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
1801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
1861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
1921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
1981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
2041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
2101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
2161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
2641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
2701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
2761 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
2821 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
2881 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
2941 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
3001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3061 ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc tgtcaaatgg
3121 acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg
3181 ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
4081 cgccattcag ag
//