LOCUS Exported 7115 bp ds-DNA circular SYN 05-NOV-2016
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..7115
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 332..349
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 353..385
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 389..412
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
CDS 431..3478
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/note="lacZ"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
terminator 3685..3771
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3863..3890
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 3910..4001
/gene="bla"
/note="AmpR promoter"
CDS 4002..4862
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5033..5621
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5807..5947
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(6211..7089)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
301 ctaacaggag gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag
361 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccaat
421 gatagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
481 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
541 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggt
601 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
661 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta
721 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
781 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
841 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
901 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
961 ggtgatggtg ctgcgttgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
1021 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
1081 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
1141 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
1201 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
1261 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
1321 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
1381 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
1441 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
1501 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
1561 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
1621 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
1681 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
1741 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
1801 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
1861 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
1921 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
1981 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
2041 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
2101 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
2161 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
2221 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
2281 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
2341 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
2401 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
2461 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
2521 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
2581 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
2641 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
2701 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
2761 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
2821 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
2881 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
2941 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
3001 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
3061 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
3121 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
3181 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
3241 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
3301 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
3361 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
3421 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataagc
3481 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
3541 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
3601 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
3661 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
3721 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
3781 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
3841 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
3901 caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
3961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
4021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
4081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
4141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
4201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
4261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
4321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
4381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
4441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
4501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
4561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
4621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
4681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
4741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
4801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
4861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
4921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
4981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
5041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
5101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
5161 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
5221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
5281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
5341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
5401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
5461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
5521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
5581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
5641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
5701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
5761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
5821 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
5881 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
5941 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
6001 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
6061 accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca
6121 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
6181 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
6241 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
6301 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
6361 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
6421 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
6481 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
6541 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
6601 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
6661 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
6721 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
6781 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
6841 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
6901 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
6961 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
7021 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
7081 ttcagccata cttttcatac tcccgccatt cagag
//