質(zhì)粒簡介
pBAD30是一個(gè)大腸桿菌pBAD系列表達(dá)質(zhì)粒。
質(zhì)粒序列
LOCUS Exported 4919 bp ds-DNA circular SYN 12-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS pBAD30
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4919)
TITLE Direct Submission
JOURNAL Exported Monday, September 12, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4919
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1001..1285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 1306..1362
/note="MCS"
/note="pUC18/19 multiple cloning site"
terminator 1565..1651
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1743..1770
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1789..1880
/gene="bla"
/note="AmpR promoter"
CDS 1881..2741
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2783..3238
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin complement(4199..4744)
/direction=LEFT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
2461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
2521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
2581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
2641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
2701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
2761 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
2821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
2881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
2941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
3001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
3061 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc
3121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
3181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
3241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
3301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtcaggcatt tgagaagcac
3601 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct
3661 atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat
3721 tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact
3781 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
3841 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
3901 cagcaccttg tcgccttgcg tataatattt gccgctagcg gagtgtatac tggcttacta
3961 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
4021 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
4081 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
4141 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
4201 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
4261 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
4321 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
4381 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
4441 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
4501 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
4561 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
4621 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
4681 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
4741 tcaagaagat catcttatta atcagataaa atatttgctc atgagcccga agtggcgagc
4801 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
4861 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc
//