質粒序列
LOCUS Exported 6179 bp ds-DNA circular SYN 17-9-2015
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6179)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-9-17 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6179
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(191..1069)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1215..1380
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
terminator 1664..1750
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1842..1869
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(1950..1966)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(2489..3280)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKEVAADWAMERLPAQYQP
VIREARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
CDS complement(4314..5264)
/codon_start=1
/gene="rep101"
/product="protein needed for replication with the pSC101
origin"
/note="Rep101"
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 5312..5534
/note="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
protein_bind 6099..6120
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6135..6165
/note="lac promoter"
/note="promoter for the E. coli lac operon"
ORIGIN
1 cctgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
61 ggcgtagagg atctgctcat gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa
121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
1021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
1081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
1141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
1201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
1261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
1321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
1381 acccgttttt ttgggctagc aggaggaatt caccatggta cccggggatc ctctagagtc
1441 gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata
1501 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
1561 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
1621 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
1681 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
1741 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
1801 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
1861 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt
1921 atccgctcat gagacaataa ccctagctta ctggccgtcg ttttacaacg tcgtgactgg
1981 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
2041 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
2101 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
2161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
2221 ccaacacccg ctgacgagct tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat
2281 tacttcgcca actattgcga taacaagaaa aagccagcct ttcatgatat atctcccaat
2341 ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt
2401 ggctgtgagc aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc
2461 ggcgtcggct tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt
2521 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
2581 gtccaagata agcctgtcta gcttcacgta tgacgggctg atactgggcc ggcaggcgct
2641 ccattgccca gtcggcagcg acctccttcg gcgcgatttt gccggttact gcgctgtacc
2701 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
2761 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
2821 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
2881 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
2941 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
3001 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
3061 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
3121 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
3181 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
3241 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag
3301 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
3361 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat
3421 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
3481 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg
3541 tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca
3601 gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca
3661 cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc
3721 tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga
3781 ccccggatga agtaattccc acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc
3841 tagtttgtta tcagaatcgc agatccggct tcaggtttgc cggctgaaag cgctatttct
3901 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
3961 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
4021 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
4081 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
4141 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
4201 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
4261 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
4321 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
4381 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
4441 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
4501 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
4561 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
4621 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
4681 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
4741 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
4801 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
4861 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
4921 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
4981 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
5041 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
5101 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
5161 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
5221 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
5281 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
5341 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
5401 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
5461 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
5521 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
5581 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
5641 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
5701 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
5761 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
5821 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
5881 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
5941 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
6001 ccgtcttact gtcgggaatt aattcgttgg ccgattcatt aatgcagctg gcacgacagg
6061 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
6121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgag
//