質(zhì)粒序列
LOCUS Exported File 3162 bp ds-DNA circular SYN 25-11-2013
DEFINITION Cloning vector with a phage origin for producing single-stranded
DNA. The MCS is reversed in pUC119.
ACCESSION U07649
VERSION .
KEYWORDS pUC118
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3162)
AUTHORS Vieira J, Messing J.
TITLE Production of single-stranded plasmid DNA.
JOURNAL Meth. Enzymol. 1987;153:3-11.
PUBMED 3323803
REFERENCE 2 (bases 1 to 3162)
AUTHORS TaKaRa
TITLE Direct Submission
JOURNAL Exported 2015-5-18 from MLCC
http://www.miaolingbio.com
COMMENT
FEATURES Location/Qualifiers
source 1..3162
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
rep_origin complement(187..642)
/direction=LEFT
/note="M13 ori"
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(619..945)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of?beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRP
VAAH"
primer_bind 855..871
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 875..931
/gene="
"
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(941..957)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 965..981
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(989..1019)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1343..1931)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2102..2962)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2963..3067)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
241 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc
301 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
361 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
421 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
481 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
541 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
601 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg
661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
841 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
901 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt
961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
1021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
1081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
1141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
1201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
1261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
1321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
1381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
1441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
1501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
1561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
1621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
1681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
1741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
1801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
1861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
1921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
1981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
2041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
2101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
2161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
2221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
2281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
2341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
2401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
2461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
2521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
2581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
2641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
2701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
2761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
2821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
2881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
2941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
3001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
3061 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
3121 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//