pACYC184質(zhì)粒是一種原核表達載體,含有p15A復(fù)制起點的大腸桿菌質(zhì)??寺≥d體。這允許pACYC184在與ColE1相容性組的質(zhì)粒(例如pBR322,pUC19)的細(xì)胞共存。它是一個低拷貝數(shù)載體,每個細(xì)胞約15個拷貝,但可以用壯觀霉素放大。由于cat基因的存在,氯霉素不能用于擴增。
pACYC184 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC184 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplifed with spectinomycin. Chloramphenicol cannot be used for amplifcation due to the presence of the cat gene.