間變型**瘤激酶抗體

規(guī)格：1mg/1ml

英文名： ALK

別名： ALK tyrosine kinase receptor; ALK tyrosine kinase receptor precursor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Ana

分子量： 174kDa

儲(chǔ)存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

克隆類型：Polyclonal

亞型：IgG

純化方法：affinity purified by Protein A

**原：KLH conjugated synthetic peptide derived from human CD246 N-

交叉反應(yīng)：Human, Mouse, Rat, Dog, Cow, Horse,

間變型**瘤激酶抗體細(xì)胞定位：細(xì)胞膜

產(chǎn)品介紹：background: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), LK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]. Function: Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a間變型**瘤激酶抗體 secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. Subunit: Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. Tissue Specificity: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. Post-translational modifications: Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. N-glycosylated. DISEASE: Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15)間變型**瘤激酶抗體 with CARS; translocation t(2;4)(p23;q21) with SEC31A. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 1 LDL-receptor class A domain. Contains 2 MAM domains. Contains 1 protein kinase domain. Gene ID: 238 Database links: Entrez Gene: 238 Human Entrez Gene: 11682 Mouse Entrez Gene: 266802 Rat Omim: 105590 Human SwissProt: Q9UM73 Human SwissProt: P97793 Mouse Unigene: 654469 Human Unigene: 311854 Mouse Unigene: 201918 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ALK蛋白在細(xì)胞生長(zhǎng)調(diào)控中起重要作用，主要表達(dá)在神經(jīng)細(xì)胞,白細(xì)胞癌基因蛋白。 ALK p80是多向性生長(zhǎng)因子酪氨酸激酶受體蛋白， 間變性大細(xì)胞**瘤伴有t(2;5) (p23;q35)染色體的易位，易位后的細(xì)胞基因表達(dá)分子量為80kD，常伴有CD30陽(yáng)性，ALK p80陽(yáng)性的間變性大細(xì)胞**瘤預(yù)后好于陰性病例。 主要用于間變性大細(xì)胞**瘤與何杰金氏**瘤的鑒別診斷，間變性大細(xì)胞**瘤中陽(yáng)性率大約為70%左右，還可以用于骨髓中間變性大細(xì)胞**瘤的診斷.

間變型**瘤激酶抗體產(chǎn)品應(yīng)用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

研究領(lǐng)域：**學(xué)  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  生長(zhǎng)因子和**  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  細(xì)胞類型標(biāo)志物  b-**細(xì)胞  腫瘤細(xì)胞生物標(biāo)志物  

儲(chǔ)存條件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

來(lái)源： Rabbit

外觀： Lyophilized or Liquid

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