5-溴脫氧尿嘧啶核苷/增殖標志物單克隆抗體
規(guī)格：1mg/1ml
英文名： BrdU
別名： Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
分子量： 0.3071kDa
英文名： BrdU
別名： Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
分子量： 0.3071kDa
儲存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆類型：Monoclonal
亞型：IgG
純化方法：affinity purified by Protein G
**原：bromodeoxyuridine conjugated to KLH
交叉反應(yīng)：BrdU
5-溴脫氧尿嘧啶核苷/增殖標志物單克隆抗體細胞定位：細胞核
產(chǎn)品介紹：background: Proliferation Marker Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity 5-溴脫氧尿嘧啶核苷/增殖標志物單克隆抗體of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988),5-溴脫氧尿嘧啶核苷/增殖標志物單克隆抗體 tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. CAS: 59-14-3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 增殖標志物 BrdU（溴化脫氧尿嘧啶核苷）可以在體內(nèi)和體外摻入到處于S期的細胞所合成的DNA鏈中。此抗體可以與摻入到任何種屬細胞中的BrdU反應(yīng)，與碘脫氧尿嘧啶有交叉反應(yīng)，標記摻有BrdU的S期細胞，主要用于研究各種不同的因素對正常/腫瘤組織的細胞增殖及動力學的研究。 用FITC標記的抗BrdU IgG可用于流式細胞術(shù)定量檢測增值細胞。
5-溴脫氧尿嘧啶核苷/增殖標志物單克隆抗體產(chǎn)品應(yīng)用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究領(lǐng)域：細胞生物 **學 發(fā)育生物學 染色質(zhì)和核信號 神經(jīng)生物學 **及化合物 細胞類型標志物
儲存條件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
來源： Mouse
外觀： Lyophilized or Liquid
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