表達(dá)區(qū)間:chain a+chain b 分子量:5.8 kDa 標(biāo)簽:No tag 純度:Greater than 98% as determined by SDS-PAGE 內(nèi):≤5 EU/mg 應(yīng)用:The concentration in serum-free medium is generally 5-20 mg/L, please check out the optimal concentration for different cell lines. 活性:29 IU/mg 性狀:Lyophilized powder 溶解方法:Reconstitute at 1-2 mg/ml in 10 mM HCl by gentle pipetting 2-3 times, don’t vortex 存儲(chǔ):The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at -20°C for up to 6 months after 0.22 μm filtered. Avoid repeated freeze/thaw cycle. 別名:IDDM Protein, IDDM1 Protein, IDDM2 Protein, ILPR Protein, ins Protein, INS_HUMAN Protein, Insulin A chain Protein, Insulin B chain Protein, IRDN Protein, MODY10 Protein, Preproinsulin Protein, Proinsulin Protein, Proinsulin precursor Protein
重組蛋白的制備方法:
● 表達(dá)系統(tǒng)選擇:選擇適合的表達(dá)系統(tǒng)是重組蛋白制備的關(guān)鍵一步。常用的表達(dá)系統(tǒng)包括大腸桿菌、酵母、昆蟲(chóng)細(xì)胞和哺乳動(dòng)物細(xì)胞。每種表達(dá)系統(tǒng)都具有不同的優(yōu)缺點(diǎn),適用于不同類型的蛋白質(zhì)表達(dá)。
蛋白特點(diǎn):
重組蛋白用途及應(yīng)用: