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Apa I FDRE1124

產(chǎn)品價(jià)格: < 面議
產(chǎn)品型號(hào):FDRE1124
 牌:Vivantis Technologies Sdn. Bhd.
公司名稱:上海拜力生物科技有限公司
  地:上海閔行
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產(chǎn)品簡(jiǎn)介

Home  Restriction Endonucleases  Fast Digest Restriction Endonucleases  Apa I
Apa I
Concentration
20u/μl 

5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

產(chǎn)品詳細(xì)信息

Home  Restriction Endonucleases  Fast Digest Restriction Endonucleases  Apa I

Apa I

Concentration
20u/μl 
5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37?C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65?C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 20-fold overdigestion with Apa I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Apa I for 16 hours at 37?C.

Ordering Information

Catalog No Description Pack Size
FDRE1124 Apa I 50 preps

 

Downloads
Manual

Apa I

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