產品介紹
品名	Guinea pig antiserum against parvalbumin
貨號	GP72
規(guī)格	100 μl
產品形式	lyophilized antiserum. No preservatives.
復溶	with 100 μl of bi-distilled water.
克隆性	polyclonal
反應性	human
宿主動物	Guinea-pig
抗體形式	Antiserum lyophil.
描述	This antibody was raised against recombinant mouse parvalbumin. GP72 reacts specifically with parvalbumin on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (Fig. 1). The antibody specifically localizes parvalbumin using free-floating or mounted sections of brain (Fig. 2), kidney and muscles of probably all vertebrates The antiserum does not stain the brain of parvalbumin-KO mice. Spectacular staining found in immunohistochemistry on floating sections of 4% PFAfixed specimens.
稀釋	Immunohistochemistry: 1:3'000 - 1:10'000, when performed with the avidin-biotin method.  Western blots: 1:1’000 to detect parvalbumin in brain extracts. We recommend that the optimal dilutions be determined by titration experiments.
儲存	For long storage keep small aliquots of the reconstituted antiserum (e.g. 1 μl) at -80°C (or at least at -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freeze-thaw cycles.
參考文獻	1. Filice F., Celio, M.R., Szabolcsi V. (2017) JCN, in press